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<meta content="RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth  " name="eprints.title" />
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<meta content="In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), Harnessing and Exploiting Global Opportunities’, Proceedings of the
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<meta content="All organisms are subject to mutations as a result of normal cellular operations or interactions with the environment, leading to genetic variation (polymorphism). In conjunction with selection and genetic drift, there arises genetic variation within and among individuals and species. For this variation to be useful to geneticists, it must be heritable and discernable whether as a recognisable phenotypic variation or as a genetic mutation distinguishable through molecular techniques. PCR amplification and gel electrophoresis resolution of products using RAPD primer A2 shows polymorphism of up to 5 bands was evident. It also demonstrates that at the molecular level, there are banding differences that can be picked up between crossbred progeny sired by rams with high genetic merit for growth or muscle.Fat depth at the GR and C sites was significantly (P<0.05) influenced by level of nutrition and nutrition level x siretype interaction in that fat depths were greater in crossbred lambs fed at high levels of nutrition than those fed low nutrition levels. Lambs selected for growth and fed high level of nutrition produced carcasses with the least KNIFE GR fat depth of 11.4mm, a significant reduction from 18.1mm in the control group fed low level nutrition. " name="eprints.abstract" />
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<meta content="1. Hegarty, R. S., Shands, C., Marchant, R., Hopkins, D. L., Ball, A., Harden, S. (2006). Effects of available nutrition and sire breeding values for growth and muscling on the development of crossbred lambs 1. Growth and carcass characteristics. Australian Journal of Agricultural Research in press.
2. Liu, Z. J., Cordes, J. F. (2004). DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238: 1-37.

3. Malau-Aduli, A. E. O., Bignell, C.W., Tavassoli-Salardini, F., Smolenski, A. J., Palmer, A., Bignell, J., 
Burbury, S., Batchelor, R., Malau-Aduli, B. S., Adediran, S. A., Lane, P. A., Clark, R. J. (2006). Genetic  
diversity and breed comparison of carcass traits in Tasmanian Corriedale and East Friesian sheep by RAPD 
markers. Proceedings of the 52nd International Congress on Meat Science and Technology, University College
Dublin, Ireland.

4. Rabouam, C., Comes, A. M., Bretagnolle, V., Humbert, J. F., Periquet, G., Bigot, Y. (1999). Features of DNA fragments obtained by random amplified polymorphic DNA (RAPD) assays. Molecular Ecology, 8: 493-503.

5. Sambrook, J., Russell, D. W. (2001). Molecular Cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, New York.
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    <h1 class="ep_tm_pagetitle">RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Malau-Aduli, A.E.O.</span> and <span class="person_name">Bignell, C.W.</span> and <span class="person_name">Hegarty, R.S.</span> and <span class="person_name">Oddy, H.</span> and <span class="person_name">Johns, W.</span> and <span class="person_name">Tavassoli-Salardini, F.</span> and <span class="person_name">Smolenski, A.J.</span> and <span class="person_name">Malau-Aduli, B.S.</span> and <span class="person_name">Wells, B.B.</span> and <span class="person_name">Lane, P.A.</span> and <span class="person_name">Clark, R.J.</span> (2006) <xhtml:em>RAPD marker variation in meat quality traits of Poll Dorset second-cross lambs selected for muscle or growth.</xhtml:em> In: 52nd International Congress of Meat Science and Technology, 13 - 18 August 2006, Dublin, Ireland.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_3997' );" onmouseout="EPJS_HidePreview( event, 'doc_preview_3997' );" href="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a><div class="ep_preview" id="doc_preview_3997"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/2971/thumbnails/1/preview.png" border="0" class="ep_preview_image" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/2971/1/MALAU-ADULI_et_al_2006DublinPollDorsetSheep.pdf"><span class="ep_document_citation">PDF</span></a> - Requires a PDF viewer<br />197Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://www.cpregistrations.com/icomst2006/">http://www.cpregistrations.com/icomst2006/</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">All organisms are subject to mutations as a result of normal cellular operations or interactions with the environment, leading to genetic variation (polymorphism). In conjunction with selection and genetic drift, there arises genetic variation within and among individuals and species. For this variation to be useful to geneticists, it must be heritable and discernable whether as a recognisable phenotypic variation or as a genetic mutation distinguishable through molecular techniques. PCR amplification and gel electrophoresis resolution of products using RAPD primer A2 shows polymorphism of up to 5 bands was evident. It also demonstrates that at the molecular level, there are banding differences that can be picked up between crossbred progeny sired by rams with high genetic merit for growth or muscle.Fat depth at the GR and C sites was significantly (P&lt;0.05) influenced by level of nutrition and nutrition level x siretype interaction in that fat depths were greater in crossbred lambs fed at high levels of nutrition than those fed low nutrition levels. Lambs selected for growth and fed high level of nutrition produced carcasses with the least KNIFE GR fat depth of 11.4mm, a significant reduction from 18.1mm in the control group fed low level nutrition. </p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Conference or Workshop Item (Paper)</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">In: D. Troy, R. Pearce, B. Byrne, J. Kerry (Editors), Harnessing and Exploiting Global Opportunities’, Proceedings of the&#13;
52nd International Congress of Meat Science and Technology, University College Dublin, Ireland, 52:89-90 (2006)</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">RAPD markers, Poll Dorset crossbred lambs, meat quality, growth, muscle</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270200.html">270000 Biological Sciences &gt; 270200 Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300401.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300400 Animal Production &gt; 300401 Animal Breeding</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270208.html">270000 Biological Sciences &gt; 270200 Genetics &gt; 270208 Molecular Evolution</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300400.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300400 Animal Production</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270800.html">270000 Biological Sciences &gt; 270800 Biotechnology</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2971</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Aduli E.O. Malau-Aduli</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">24 Jan 2008 12:10</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">24 Jan 2008 12:10</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2971;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2971">item control page</a></p>
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